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FACS Sorting Aria Specs

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BDFACSAria

The Biomedical Sciences Flow Cytometry Core Laboratory uses a BD-Biosciences FACS Aria high speed flow cytometer/cell sorter utilizing a quartz cuvette for interrogation. It is equipped with three diode lasers providing excitation lines at 488 nm, 633 nm and 407 nm. Signal processing is digital, allowing high acquisition rates, recapitulation and logical analysis of acquired data. There are 5 emission wavelengths detected from 488 nm excitation, 3 from 633 nm excitation and 3 from 407 nm excitation to accommodate a wide range of fluorochromes. The Aria is capable of high speed cell sorting (>30,000 events/second) and collecting sorted samples into up to four 2ml or 5ml tubes, two 15ml tubes and is also equipped with a robotic device for sorting into multi-well plates and onto glass slides.

BDLSRII

The Biomedical Sciences Flow Cytometry Core Laboratory has access to a BD-Biosciences LSRII Flow Cytometer also utilizing a quartz cuvette for interrogation. It is equipped with three lasers providing excitation lines at 488nm, 633nm and the UV line at 325nm. Signal processing is digital, allowing high acquisition rates, recapitulation and logical analysis of acquired data. There are 4 emission wavelengths detected from 488 nm excitation, 2 from 633 nm excitation and 2 from 407 nm excitation to accommodate a wide range of fluorochromes.