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| Using The Facility |
| Contact Us |
| Location |
| FACS Sorting Aria Specs |
| BD LSR II Specs |
| Protocols | Sample prep | Rates | Cytometry Software | Yen Lab | Other Cornell Cytomtery Resources |
| All Runs Require | Negative unstained controls (0.5ml minimum volume) |
Compensation controls for each fluor (0.5ml minimum volume) |
| Acquisition Samples |
It is nice to have 0.5ml of sample volume with 1million cells per ml. | Data acquisition runs typically only acquire 10,000 to 30,000 gated events, but still require a minimum 250ul of sample volume. |
| Sort Runs | Cells should be at 20-30 million cells/ml (5,000 to 35,000 events/s) |
The Aria can sort at up to 50- 100 million cells/hour (a flow rate of 0.2-0.8ul/s and up to 2ul/s with less resolution) |
| Sort Time | ~1-2 ml of sample volume per hour. Collection rate(cells/hour) = 2(%Viability) X (%Positives) X (cell concentration) |
Collection time = (Sort number target) X (collection rate) * not including set-up |
| Collection Tubes | Investigator should bring collection tubes |
1ml, 5ml, or 15ml tubes- some cells require tubes to be coated with BSA/Serum or sorted into tubes containing media |
| Collection Tubes | 1ml microtubes-Bio-Rad-223-9391 5ml (12X75mm)-BD Biosciences-352058 15ml conical-BD Biosciences-352196 |
Multiwell plates and glass slides 96,48,12,6 well plates max 1.5cm in height slides should be coated |
| Questions | Please direct all questions to Jim at (607)253-4004 biomedflowcore@cornell.edu | |
| Directions | The facility is located in the basement of the Vet.
Research Tower down the far right corridor and around the left corner. |
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