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Cytometry Resources

The Biomedical Sciences Flow Cytometry Core Laboratory at Cornell University located in Ithaca NY provides a wide variety of cytometry services for investigators at Cornell University as well as external investigators. We currently have access to a hi-speed FACSAria cell sorter and an LSR II. We will be happy to help you establish or convert your assay to flow cytometry, as well as aid in the analysis and interpretation of flow cytometry data.

Flow Cytometry allows the rapid acquisition of data from thousands to millions of particles. Each particle is measured for relative size, internal complexity and relative fluorescence intensity for up to 12 colors. We currently analyse samples from a wide range of sources from yeast and fungus, to the fly, rodents, pets and other larger animals. An example of some common assays we are currently running are shown below.

New Investigators:
We ask that all new investigator fill out a cytometry request form the first time they use the facility. Users with established protocols can just email us with the run information. New users should estimate run time and prepare appropriate controls- see our sample prep page.

This core facility does not currently support unassisted use of these core instruments, we will run and sort the samples for you. There are other fee for use CU cytometry related instruments available on the CU campus.

The fees for use of the facility are:
Vet. College FACS- $40.00/hr plus a 1hr set-up charge for sorting runs.
Vet College analysis-$40.00/hr

Cornell University(non Vet.) FACS-$60.00/hr
Cornell University(non Vet.) Analysis-$60.00/hr

Non Cornell FACS-$60.00/hr
Non Cornell Analysis-$60.00/hr

All Runs Require Negative unstained controls
(0.5ml minimum volume) Compensation controls for each fluor
(0.5ml minimum volume)

Acquisition
Samples It is nice to have 0.5ml of sample volume with 1million cells per ml. Data acquisition runs typically only acquire 10,000 to 30,000 gated events, but still require a minimum 250ul of sample volume.

Sort Runs Cells should be at 20-30 million cells/ml
(5,000 to 35,000 events/s) The Aria can sort at up to 50- 100 million cells/hour
(a flow rate of 0.2-0.8ul/s and up to 2ul/s with less resolution)

Sort Time ~1-2 ml of sample volume per hour.
Collection rate(cells/hour) = 2(%Viability) X (%Positives) X (cell concentration) Collection time = (Sort number target) X (collection rate)
* not including set-up

Collection Tubes Investigator should bring collection tubes
1ml, 5ml, or 15ml tubes- some cells require tubes to be coated with BSA/Serum or sorted into tubes containing media

Collection Tubes 1ml microtubes-Bio-Rad-223-9391
5ml (12X75mm)-BD Biosciences-352058
15ml conical-BD Biosciences-352196 Multiwell plates and glass slides
96,48,12,6 well plates max 1.5cm in height
slides should be coated

Questions Please direct all questions to Jim at (607)253-4004 biomedflowcore@cornell.edu

Directions

The facility is located in the basement of the Vet. Research Tower down the far right corridor and around the left corner.

All samples with large amount of debris, dead cells or particulate material must be filtered through nylon mesh of less than 70microns.

A charge of $2.00/sterile filter for samples filtered at the facilty or Cell filters can be purchased at:

Thomas Scientific Sterile 40micron-50ml tube BD cell strainers 4620E99 $87.40/cs50

Thomas Scientific Sterile 70micron-50ml tube BD cell strainers 4620F02 $87.40/cs50

Thomas Scientific non-Sterile 70micron-6ml 12X75mm tubes 4620F40 $447.70/cs100

SEFAR America inc.
1-800-995-0531 NONsterile 41micron-sheets
(Autoclave safe) NITEX 03-41/31 $179.04/3 yards

All Runs Require	Negative unstained controls (0.5ml minimum volume)	Compensation controls for each fluor (0.5ml minimum volume)
Acquisition Samples	It is nice to have 0.5ml of sample volume with 1million cells per ml.	Data acquisition runs typically only acquire 10,000 to 30,000 gated events, but still require a minimum 250ul of sample volume.
Sort Runs	Cells should be at 20-30 million cells/ml (5,000 to 35,000 events/s)	The Aria can sort at up to 50- 100 million cells/hour (a flow rate of 0.2-0.8ul/s and up to 2ul/s with less resolution)
Sort Time	~1-2 ml of sample volume per hour. Collection rate(cells/hour) = 2(%Viability) X (%Positives) X (cell concentration)	Collection time = (Sort number target) X (collection rate) * not including set-up
Collection Tubes	Investigator should bring collection tubes	1ml, 5ml, or 15ml tubes- some cells require tubes to be coated with BSA/Serum or sorted into tubes containing media
Collection Tubes	1ml microtubes-Bio-Rad-223-9391 5ml (12X75mm)-BD Biosciences-352058 15ml conical-BD Biosciences-352196	Multiwell plates and glass slides 96,48,12,6 well plates max 1.5cm in height slides should be coated
Questions	Please direct all questions to Jim at (607)253-4004 biomedflowcore@cornell.edu
Directions	The facility is located in the basement of the Vet. Research Tower down the far right corridor and around the left corner.

All samples with large amount of debris, dead cells or particulate material must be filtered through nylon mesh of less than 70microns.
A charge of $2.00/sterile filter for samples filtered at the facilty or Cell filters can be purchased at:
Thomas Scientific	Sterile 40micron-50ml tube BD cell strainers	4620E99	$87.40/cs50
Thomas Scientific	Sterile 70micron-50ml tube BD cell strainers	4620F02	$87.40/cs50
Thomas Scientific	non-Sterile 70micron-6ml 12X75mm tubes	4620F40	$447.70/cs100
SEFAR America inc. 1-800-995-0531	NONsterile 41micron-sheets (Autoclave safe)	NITEX 03-41/31	$179.04/3 yards