Dr. Klaus W. Beyenbach
Professor of Physiology
Department of Biomedical Sciences
VRT 8004
Cornell University
Ithaca, N.Y. 14853Phone: (607) 253-3482
FAX: (607) 253-3851
E-MAIL: KWB1@CORNELL.EDU
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Dr. Klaus W. Beyenbach |
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ADDITIONAL APPOINTMENTS
Cornell University Director, Program of Study 'Animal Physiology', Biological Sciences, Cornell University National/International Guest Professor, Department of Biology/Chemistry, University of Osnabrueck, Osnabrueck, Germany |
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PERSONAL DATA
Born 19 March 1943, Mainz, Germany |
EDUCATION
1968, B.A. Biology (minor Chemistry) 1970, M.A. Biology (minor Biochemistry) 1974, Ph.D. Zoophysiology (minor Biochemistry) |
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PROFESSIONAL EXPERIENCES
1968-1970, Teaching Assistant 1971-1974, NIH Predoctoral Trainee 1974-1976, Fellow, National Kidney Foundation 1976-1978, NIH Fellow 1978-1982, Assistant Professor of Physiology 1982-1988, Associate Professor of Physiology 1985-1986, Visiting Professor 1991-1992, Visiting Professor 1994 - 1997, Vice President 1997 - 1999, President 1978 - 2002, Graduate Faculty, Field of Physiology, 1989 - present, Professor of Physiology 1999 - 2001, Chair, Epithelial Transport Group, 1999 - present, Director of Undergraduate Physiology 2002 - present, Graduate Faculty, Field of Comparative Biomedical 2006 - present, Graduate Faculty, Field of Toxicology, Cornell |
AWARDS (continuous external research support since 1974)
1969-1970, NSF Predoctoral Fellow, National Science Foundation 1971, Predoctoral Scholarship Marine Science Institute, University of Texas, Port Aransas, Texas, USA 1971-1974, Predoctoral NIH Fellowship, National Institutes of Health 1975-1976, Postdoctoral Fellowship, National Kidney Foundation 1976-1978, Postdoctoral Fellowship, National Research Council, NIH 1980-1984, Principal Investigator, Mt. Desert Island Biological Laboratory Salisbury Cove, ME 1985, International Fellow, Fogarty Center, National Institutes of Health 1991, Alexander-von-Humboldt-Preis, Alexander-von-Humboldt-Stiftung, Bonn, Germany 1995, Resident Fellow, Bellagio Study and Conference Center, Rockefeller Foundation, Bellagio, Italy 1997, Honorary Member, Golden Key National Honor Society (student nomination) 1999, Bundesverdienstkreuz 1. Klasse, Officer's Cross of the German National Order of Merit (highest German civilan award) 2003, Mercator Award, Deutsche Forschungsgemeinschaft, Bonn, Germany |
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BOARDS
1995 - 2001, Editorial Board, American Journal of Physiology, Regulatory Integrative Comparative Physiology 1995 - 2001, Board of Directors, Alexander von Humboldt Association of America 1998 - present, Editorial/Advisory Board, Progress in Fish Research 1998 - present, Editorial Board, Journal of Insect Physiology 2000 - present, Editorial Board, Insect Biology (SPARC) |
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RESEARCH INTERESTS
Mechanism and regulation of epithelial transport, function of the kidneys, aglomerular urine formation, secretory transport across insect Malpighian tubules, magnesium transport across biological membranes Design of living systems, emerging properties in biological systems |
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ACTIVE SCIENTIFIC COLLABORATIONS
Dr. Sheng Zhang, Director of Proteomics and Mass Spectrometry, Biotechnology, Cornell University Mr. John Hunt, Dept. Materials Sciences and Engineering, Cornell University; Quantitative X-ray spectroscopy, Electron probe. Dr. David A. Schooley, Department of Biochemistry, University of Nevada, Reno, NV; Mechanism of action of antidiuretic peptides in insect Malpighian tubules. Dr. Helmut Wieczorek, Department of Biology/Chemistry, University of Osnabrueck, Germany; Regulation of the V-type H+-ATPase in insect Malpighian tubules. |
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For the last 50 years, the Ussing model (left) has laid the conceptual foundation for investigating and understanding transepithelial transport. Originally conceived to explain Na+ absorption from pond water (yellow) across the skin of the frog, the model has been extended to explain epithelial transport of all sorts of solutes. Central to the Ussing model is the Na/K pump that generates intracellular concentations, high (red) for K+ and low for Na+. Transmembrane K+ and Na+ gradients are subsequently used to generate membrane voltages via channels and to drive Na+- and K+-dependent carriers (not shown). Thanks to the work of William Harvey (Univ. Florida) and Helmut Wieczorek (Univ. Osnabrueck, Germany), we now realize that membranes can also be energized by the V-type H-ATPase (yello), a proton pump (right). The ATP-driven H+-pump generates large voltages (>200 mV) and proton gradients across the cell membrane it inhabits. The proton gradient is used to driven H+-driven carriers, such as K/H exchange (blue). The voltage is used to drive paracellular Cl transport and K entry into the cell across the basolateral membrane on the other side of the cell. |
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OUR PRESENT LABORATORY
Klaus W. Beyenbach, PhD Peter M. Piermarini, PhD Laura F. Grogan, Leadership student, Summer 2007, Veterinary student from the U. of Sidney Faculty of Veterinary Science Austin W. Blum, undergraduate premed student, APS Fellow Summer 2007 Mohamed H. Khattab, undergraduate premed student Kenneth Lau, undergraduate premed student Stephen A. Schepel, undergraduate premed student Tiffany Sou, undergraduate premed student Atsuko Yamahiro, undergraduate premed student |
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| Dr. Peter Piermarini, Post-doctoral fellow. Dr. Piermarini aims to identify the proteins (transporters) involved in acid/base transport by Malpighian tubules. He is intensely curious about biological mechanisms and unusually generous with his mind and time. He has the gift of friendship and the promise of greatness. | Laura Grogan, Leadership Student, visiting veterinary student, U. Sydney. In the short time of 10 weeks during the summer of 2007 Ms. Grogan has cloned the gene of the first Cl/HCO3 exchanger in a Malpighian tubule. Here is a future veterinarian/scientist from whom we can expect important contributions to her profession. | Austin Blum, Cornell premedical student, Summer Fellow 2007, American Physiological Society. The multitalent of Mr. Blum has him pursue the perfect experiment, philosophy (from Copernicus to post-modernism) and Cl- transport across basolateral membranes of principal cells in Malpighian tubules. Erudite and brilliant; he will probably pursue a career in academic medicine. |
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| Kenneth Lau, Cornell premedical student. Joining the lab to help us mass-rear mosquitoes for proteomic studies, he quickly extended his 'service' to include research. His great teaching skills rest on his solid understanding of the subject. A future physician/scientist for the three worlds in medicine: 'Service, Teaching, and Research' in a medical school of his choice, no doubt. Understatement and a charming smile carry his winning personality. | Stephen Schepel, Cornell Junior, premedical student. His Can-Do personality has real foundations in native intelligence, manual dexterity, unusual determination and great common sense. It is hard to imagine where his potential for growth will take him. | Tiffany Sou, Cornell premedical student, Junior. She entered the lab in the summer of 2007 and advanced the definition of 'efficiency' to new heights of meaning. She holds the lab record for 1) learning the Ramsay method (thanks to Stephen and Andrew), and 2) earning co-authorship on a paper. Focused, intensely private and smart. |
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| Atsuko Yamahiro, Cornell premedical student, Senior, who is as thoughful and meticulous in her personal relationships as she is in the execution of experiments, producing the clearest of results. Kind, caring and disciplined, she is the female equal to Dr. Rieu (of Albert Camus). Reserved and elegant, with uncommon grace. She has been selected as teaching assistant for medical students attending the Cornell Medical College in Qatar. | ||
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RECENT GRADUATES OF THE LABORATORY
Xinghe Weng, BS Physiology 1996, MS Physiology 2000, Peking University; PhD Cornell University, 2007. Ming-Jiun (Brad) Yu, BS Zoology 1993, MS Physiology 1995, National Taiwan University; PhD Cornell University, 2004. Andrew Fox, BS Biology, Cornell University, 2004. Ankit Patel, BS Biology, Cornell University, 2004. Richard Massaro, BS Biology, Cornell University, 2004. Daniel S. Wu, BS Biology, Cornell University, 2003. Isabel Rodriguez-Barraquer, MD, University of Bogota, Columbia, 2005. |
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| XingHe Weng; PhD 2007, Present Postion: Post-doc, University of Utah |
Ming-Jiun Yu; PhD 2004, Present Postion: Post-doc, National Institutes of Health |
Ankit Patel; BS 2004, Present Postion: Md/PhD student, Cornell Weill Medical College |
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| Andrew Fox; BS 2004, Present Postion: Veterinary student, Ross University School of Veterinary Medicine |
Richard Massaro; BS 2004, Present Postion: Graduate student, Forensic Medicine Program, Drexel University, Philadelphia, PA |
Daniel Wu; BS 2003, Present Postion: Medical student, Duke University |
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| Isabel Rodriguez-Barraquer; MD 2005, Present Postion: Graduate student, PhD candidate, Epidemiology, Johns Hopkins University |
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PUBLICATIONS
Book Chapters (since 2000) Beyenbach, K.W. Magnesium Transport Across Cell Membranes: A Hypothesis. Proceedings of the 9th International Magnesium Symposium, Vichy, France, Sept. 2000, Proceedings of Mag 2000 : "Advances in Magnesium Research : Nutrition and Health" (Y. Rayssiguier, A. Mazur and J. Durlach eds.), John Libbey & Co Ltd, London, pp. 23 - 37, 2001. Beyenbach, K.W. and H.H. Hagedorn. Osmotic and ionic regulation by mosquitoes. In: Biology of Disease Vectors. William C. Marquardt, editor, Elsevier Academic Press, pp. 347-362, 2005. Invited Reviews (since 2000) Beyenbach, K.W., D. J. Aneshansley, T.L. Pannabecker, R. Masia, D. Gray, and M.-J. Yu. Oscillations of Voltage and Resistance in Malpighian tubules of Aedes aegytpi. J. Insect Physiol. 46: 321-333, 2000. Beyenbach, K.W. Renal Handling of Magnesium in Fish: From Whole Animal to Brush Border Membrane Vesicles.Frontiers in Biosciences: A Journal and Virtual Library, 5, d694-711, 2000. (http://www.bioscience.org/current/vol5.htm) Beyenbach, K.W. Energizing epithelial transport with the vacuolar H-ATPase. News in Physiol. Sciences, 16: 145-151, 2001. Beyenbach, K.W. Transepithelial Water Transport in Insect Malpighian Tubules. In: Perspective in Comparative Endocrinology: Unity and Diversity. H.J.Th. Gos, R.K. Rastogi, H. Vaudry, R. Pierantoni, eds., Monduzzi Editore International Proceedings Division, Bologna, Italy Chapt. pp. 713-722, 2001. Beyenbach, K.W. Regulation of Tight Junction Permeability with Switch-like Speed. Current Opinion in Nephrology and Hypertension 12: 543-550, 2003. Beyenbach, K.W. Transport Mechanisms of Diuresis in Malpighian Tubules of Insects. In: Experimental Biology of Malaria and Its Vectors. Andrew Spielman and William R. Harvey, eds., review volume, J. Exp. Biol. 206(21): 3845-3856, 2003. Beyenbach, K.W. Kidneys sans Glomeruli. Am. J. Physiol. Renal Physiol. 286: F811-F827, 2004. Beyenbach, K.W. and H. Wieczorek. The V-type H+ ATPase: Molecular Structure and Function, Physiological Roles and Regulation. J. Exp. Biol. 209: 577-589, 2006. Rajasekaran, S.A., K.W. Beyenbach and A. K. Rajasekaran. Interactions of tight junctions with membrane channels and transporters. Biochim. Biophys. Acta, Nov. 19, 2007 (Epub. ahead of print). |
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Primary Literature (since 2000)
Beyenbach, K.W., T. Pannabecker and W. Nagel. Central role of the apical membrane H+-ATPase in electrogenesis and epithelial transport in Malpighian tubules. J. Exp. Biol. 203: 1459-1468, 2000. Masia, R., D.J. Aneshansley, W.Nagel, R.J. Nachman and K.W. Beyenbach. Voltage clamping single cells in the Malpighian tubules. Am. J. Physiol. 279: F747-F754, 2000. Yu, M.J. and K.W. Beyenbach. Leucokinin and the modulation of shunt halide selectivity in Malpighian tubules. J. Insect Physiol. 47: 263-276, 2001. O’Connor, and K.W. Beyenbach. Chloride channels in apical membrane patches of stellate cells of Malpighian tubules of Aedes aegypti. J. Exp. Biol. 204: 367-378, 2001. Beyenbach, K.W. and R. Masia. Membrane conductances of principal cells in Malpighian tubules of Aedes aegypti. J. Insect Physiol. 48, 375-386, 2002. Yu, M.-J. and K.W. Beyenbach. Leucokinin activates Ca2+-dependent signal pathway in principal cells of Aedes aegypti Malpighian tubules. Am. J. Physiol. 283: F499-F508, 2002. Wu, D.S. and K.W. Beyenbach. Metabolism, electrogenesis, and conductive pathways in Malpighian tubules of Aedes aegypti. J. Exp. Biol. 206: 233-243, 2003. Weng, X.H., M. Huss, H. Wieczorek, and K.W. Beyenbach. The V-type H+ ATPase in Malpighian tubules of Aedes aegypti: Localization and activity. J. Exp. Biol. 206: 2211-2219, 2003. Yu, M.-J. and K.W. Beyenbach. Effects of leucokinin-VIII on Aedes Malpighian tubule segments lacking stellate cells. J. Exp. Biol. 207: 519-526, 2004. Scott, B.N., Yu, M.-J., Lee, L.W. and K.W. Beyenbach. Mechanisms of K+ transport across basolateral membranes of principal cells in Malpighian tubules of the yellow fever mosquito, Aedes aegypti. J. Exp. Biol. 207: 1655-1663, 2004. Massaro, R.C., Lee, L.W., Patel, A., Wu, D.S., Yu, M.J., Scott, B.N., Schooley, D.A., and K.W. Beyenbach. The mechanism of action of the antidiuretic peptide ADFa in Malpighian tubules of Aedes aegypti. J. Exp. Biol. 207: 2877-2888, 2004. Weng, X.H., K.W. Beyenbach, and A. Quaroni. Cultured monolayers of the dog jejunum with the structural and functional properties of the normal epithelium. Am. J. Physiol. Gastrointest. Liver Physiol. 288: G705-G717, 2005. Weng, X.H., Piermarini, P.M., Yamahiro, A., Yu, M.-J., Aneshansley, D.J., and K.W. Beyenbach. Gap junctions in Malpighian tubules of Aedes aegypti. J. Exp. Biol., 211:409-422, 2008. |
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ABSTRACTS/MEETINGS (since 2004)
128. Yu, M.J. and K.W. Beyenbach. Intracellular Cl- concentrations in principal cells of Malpighian tubules of the yellow fever mosquito. FASEB J. submitted, 2004. 129. Massaro, R., Schooley, D.A., and K.W. Beyenbach. Inhibitory effects of ADFa and its second messenger cGMP on transepithelial NaCl, KCl and fluid secretion but not on tubule electrophysiology in Malpighian tubules of the yellow fever mosquito Aedes aegypti. FASEB J. submitted, 2004. 130. Beyenbach, K.W. Kidneys sans glomeruli. Congress of the Deutsche Physiologische Gesellschaft. Leipzig, March 14-17, 2004. 131. Beyenbach, K.W. Transepithelial transport powered by the V-type H+-ATPase. Congress of the Deutsche Veterinärmedzinische Gesellschaft e.V. Tagung der Fachgruppe Physiologie und Biochemie. Berlin, March 26-27, 2004. 132. Beyenbach, K.W. Role of diuretic and antidiuretic peptides in extracellular fluid homeostasis in insects. Invertebrate Neuropeptide Conference 2005, Chiang Mai, Thailand, Jan. 9-13, 2005. 133. Beyenbach, K.W., X. Weng and A. Quaroni. Culture monolayers from dog jejunum with the structural and functional properties resembling the normal epithelium. BioMedical Transporters 2005: Bridging Basic and Applied Sciences. St. Gallen, Swizerland, Aug. 14-18, 2005. 144. Beyenbach, K.W. Mechanism and regulation of secretory epithelial transport in the distal segment of Malpighian tubules. Meetings of the Society of Experimental Biology (SEB), England, Barcelona, Spain, July 10-15, 2005. 145. Beyenbach, K.W. The paracellular pathway as site for the rapid, switch-like regulation of transepithelial transport in Malpighian tubules. APS Comparative Physiology Conference, Virginia Beach, Oct. 2006. 146. Weng, X., D.J. Aneshansley, and K.W. Beyenbach. Electrical coupling between principal cells of the Malpighian tubules of Aedes aegypti: measurements of the gap junction resistance. Experimental Biology 2007, Wash. DC, April 2007. 147. Weihrauch, D., P.M. Piermarini, and K.W. Beyenbach. Identification of an NHE-like protein from Malpighian tubules of the yellow fever mosquito Aedes aegypti. BioMedical Transporters 2007, Bern, Switzerland, Aug. 2007. 148. Beyenbach, K.W. On the channel-like behavior of the tight junction pathway across epithelia. 10th Symposium Signal Transduction in the Blood-Brain Barriers. Potsdam, Sanssouci, Germany, Sept. 13-16, 2007. 149. Yamahiro, A., P.M Piermarini, and K.W. Beyenbach. Identification of Na-driven anion exchanger (NDAE) splice variants from Malpighian (renal) tubules of the adult hellow fever mosquito. Exp. Biol. 2008, San Diego, CA. 150. Blum, A.W., P.M Piermarini and K.W. Beyenbach. Effects of aedeskinin and analogs on the basolateral membrane voltage (Vbl) of principal cells of Malpighian (renal) tubules of the adult yellow fever mosquito. Exp. Biol. 2008M, San Diego, CA. 151. Piermarini, P.M. and K.W. Beyenbach. Electrophysiological characterization of mosquito NHE8 in Xenopus oocytes. Exp. Biol. 2008, San Diego, CA. 152. Beyenbach, K.W. Mechanism and regulation of transepithelial Cl- secretion in Malpighian tubules of the yellow fever mosquito. SEB, Marseille, France, July 2008. 153. Baumgart, S., R. Sherwood, Celeste Ptak, Klaus Beyenbach and Sheng Zhang. 2D Gel-based proteomics analysis for insect Malpighian tubules treated with diuretic peptide aedeskinin. Assoc. Biomolec. Resource Facilities (ABRF), Salt Lake City, Utah, Feb. 2-9, 2008. |
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SEMINARS (since 2004)
The evidence for gap junctions in Malpighian tubules of the yellow fever mosquito. Universität Osnabrück, Osnabrück, Germany, Jan. 2004. The roles of the V-type H-ATPase and septate junctions in transepithelial electrolyte transport in Malpighian tubules of the yellow fever mosquito. Universität Potsdam, Potsdam, Germany, Mar. 2004. Dynamic regulation of epithelial transport via rapid changes of the paracellular, tight junction pathway. University of Leipzig, Leipzig, Germany, June 2004. New paradigms emerging from studies of epithelial transport in insect Malpighian tubules. Heinrich Heine Universität, Düsseldorf, Germany, Oct. 2004. New paradigms in epithelial transport: 1) Transport sans the Na/K ATPase, and 2) Extracellular channels. University of Vienna, Vienna, Austria, Nov. 2004. The dynamics of tight junctions. Cornell University, Ithaca, NY, Nov. 2005. The dynamics of tight junctions. Charite, Freie Universität, Berlin, Germany, Nov. 2005. The regulation of the paracellular pathway with switch-like speed. University of Arizona, Tucson, AZ, Apr. 2006. How insects flush out toxins. Cornell University, Ithaca, NY, Apr. 2006. Physiological regulation of paracellular permeation in insect epithelia: Electrophysiological evidence and molecular models. Forschungsinstitut fuer Molekulare Pharmakologie, Berlin-Buch, Germany, Sept. 2006. Hans Ussing and the mosquito. Washington State University, Pullman, WA, Oct. 2007. Powering transepithelial transport with a V-type H+ ATPase. Emory University, Atlanta, GA, Apr. 2008. |
SYMPOSIUM TALKS (since 2004)
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